Ong PY, Ohtake T, Brandt C, et al. N Engl J Med. 2002;347:1151–1160
The immune system of human skin contains several antimicrobial peptides, particularly cathelicidins (LL-37) and β-defensins. Although these peptides are negligible in normal skin, they accumulate in skin affected by inflammatory diseases such as psoriasis. The purpose of this study was to compare the levels of expression of LL-37 and human β-defensin 2 (HBD-2) in inflamed skin from patients with atopic dermatitis and from those with psoriasis.
Patients with atopic dermatitis or psoriasis were compared with normal controls with no skin disease.
The expression of LL-37 and HBD-2 protein in skin biopsy specimens was determined by immunohistochemical analysis. The amount of antimicrobial peptides in extracts of skin samples was also analyzed by immunodot blot analysis (for LL-37) and Western blot analysis (for HBD-2). Reverse transcriptase-polymerase chain reaction (RT-PCR) assays were used to confirm the relative expression of...