Egg allergy severity and persistence are associated with higher epitope-specific IgE levels to egg-white ovomucoid (Gal d 1) and immunoglobulin E (IgE) recognition of a larger number of sequential epitopes. Immunoglobulin G1, immunoglobulin G4, immunoglobulin A, and immunoglobulin D may protect against severe reactions; the epitope-specific repertoires of these isotypes were evaluated.

In the study, researchers included 38 egg-allergic children (mean age: 6.6 years; SD: 3.6; 57% boys; 63% with anaphylaxis to egg, mean egg-specific IgE: 54 kUA/L) and 6 atopic control children (mean age: 10.6 years; SD: 3.8).

A bead-based epitope assay was used to quantitate epitope-specific immunoglobulin E (esIgE), epitope-specific immunoglobulin G1 (esIgG1), epitope-specific immunoglobulin G4 (esIgG4), epitope-specific immunoglobulin A, and epitope-specific immunoglobulin D (esIgD) directed against 58 (15-mer) overlapping peptides covering the entire sequence of ovomucoid from the egg-allergic and atopic children. The relationships among epitope-specific immunoglobulins (esIgs) were evaluated by using network analysis. Linear...

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