Background: Intrapartum asphyxia accounts for the deaths of around 280,000 babies per year. Helping Babies Breathe® (HBB) is an evidence-based global neonatal resuscitation training program. HBB utilizes an in-person dissemination model. However, there are challenges to maintaining training fidelity, data collection, and continuous learning and improvement. Aim: The objective is to develop and test an integrated mobile virtual reality (VR) simulation application (eHBB) and data collection tool (mHBS) for healthcare worker training in neonatal resuscitation. Methods: eHBB, a VR training application developed on the Unreal™ platform and accessible on mobile devices and low-cost VR devices such as Google cardboard™, features three simulations in which a newborn may require only routine care, some resuscitation, or resuscitation with positive pressure ventilation. eHBB complements in-person classes, promotes individualized learning and provides standardized feedback based on the HBB Action Plan. See Figure 1. mHBS is built on the District Health Information System (DHIS2) mobile platform and features offline data collection for tracking educational and clinical outcomes using the aggregate functions of the DHIS2 database. The integrated application is available for android phones and requires no internet access for use. eHBB pilot testing was collected through surveys, direct observation, and focus group discussions. Data were analysed using descriptive statistics. Results: 24 healthcare workers participated in formal usability testing at National Hospital, Abuja, Nigeria. See Table 1. Only 9(38%) were aware of VR, none had used previously used VR. First impressions indicated that most participants found the simulations appealing and easy to use. User feedback was integrated resulting in improved usability with high task completion rates. See Figure 2. On a scale of 1 to 5 with 1 being least likely, and 5 being most likely, participants were highly likely to use the activity for practicing HBB skills (mean 4.3 ± 0.7) and to recommend it to others (mean 4.5 ± 0.6). Focus group participants had positive impressions of eHBB as interesting, providing education on best practice, helping them to remember what to do if a baby needs resuscitation, and enabling “learning without stress”. A randomized controlled trial on the feasibility, acceptability and educational effectiveness of eHBB in comparison to HBB video or control group (HBB manual only) to augment in-person classes is ongoing in Nigeria and Kenya. Conclusions: eHBB/mHBS-DHIS2 are integrated mobile VR training and data collection apps that augment neonatal resuscitation training in low and middle income countries. Mobile VR simulations may be a feasible option for introductory and refresher training in neonatal resuscitation. Acknowledgements: This project was funded by the Bill & Melinda Gates Foundation. We recognize the efforts of eHBB/mHBS project team members at the University of Washington, Indiana University, Oxford University, University of Lagos, Alupe University College, and National Hospital, Abuja.